Immunoblot:
EUROLINE: ANA Profile 3
- Differentiation of antibodies against cell nuclei (ANA).
- Indications: Sharp syndrome (MCTD), systemic lupus erythematosus, Sjögren’s syndrome, systemic sclerosis, poly/dermatomyositis, PBC.
- Serum dilution 1 : 101; conjugate class anti-human IgG, AP-labelled.
- With the EUROLINE ANA Profile 3, fifteen autoantibodies can be determined: antibodies against nRNP/Sm, Sm, SS-A, Ro-52, SS-B, Scl-70, PM-Scl, Jo-1, centromere protein B, PCNA, dsDNA, nucleosomes, histones, ribosomal P-proteins, AMA M2.
- Antibodies against SS-A are characteristic markers for SLE and Sjögren’s syndrome. In contrast, antibodies against Ro-52 also occur in patients with other autoimmune diseases.
- Native antigens, purified by affinity chromatography (exception: centromere protein B, PM-Scl, Ro-52, PCNA).
- Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster and EUROLineScan.
Auto immuneliver profile
EUROLINE: Systemic Sclerosis (Nucleoli) Profile
- Differentiation of systemic sclerosis-associated antibodies against cell-nuclear antigens.
- Indications: systemic sclerosis (SSc, diffuse and limited form), overlap syndromes.
- Serum dilution 1 : 101, conjugate class anti-human IgG, AP-labelled.
- With the EUROLINE Systemic Sclerosis Profile (Nucleoli), thirteen autoantibodies can be determined: antibodies against Scl-70, CENP A, CENP B, RP11 (RNAP-III), RP155 (RNAP-III), fibrillarin, NOR-90, Th/To, PM-Scl100, PM-Scl75, Ku, PDGFR, Ro-52.
- Test strips can be automatically incubated and evaluated using the systems EUROBlotOne, EUROBlotMaster und EUROLineScan.
Gangloside profile
EUROLINE: Profile Autoimmune Liver Diseases
- Differentiation of antibodies in autoimmune liver diseases.
- Indications: autoimmune hepatitis, primary biliary cirrhosis, overlaps syndromes.
- Serum dilution 1 : 101, conjugate class anti-human IgG, AP-labelled.
- With the EUROLINE Profile Autoimmune Liver Diseases, nine autoantibodies can be determined: antibodies against AMA M2, M2-3E (BPO), Sp100, PML, gp210, LKM-1, LC-1, SLA/LP and Ro-52.
- Test strips can be automatically incubated and evaluated using the systems EUROBlotMaster, EUROBlotOne, and EUROLineScan
EUROLINE: Paraneoplastic Neurological Syndromes 12 Ag
|
Incubated EUROLINE Neuronal Antigens Profile 12 Ag |
- Differentiation of antibodies against neuronal antigens.
- Indication: paraneoplastic neurologic syndromes (PNS).
- Serum dilution 1: 101; conjugate class anti-human IgG, AP-labelled.
- With the EUROLINE Neuronal Antigen Profile 7, twelve autoantibodies can be determined: antibodies against amphiphysin, CV2 (CRMP5), PNMA2 (Ma2/Ta), Ri, Yo, Hu, recoverin, SOX1, Titin, Zic4, GAD65 and Tr (DNER).
- Test strips can be automatically incubated and evaluated using the systems EUROBlotMaster and EUROLineScan or EUROBlotOne
EUROLINE: ganglioside profiles
- Determination of autoantibodies against gangliosides for the serological diagnosis of peripheral neuropathies.
- Membrane strips printed with individual lines of purified, biochemically characterised antigens.
- Serum dilution 1: 51; conjugate class anti-human IgG, alkaline phosphatase-labelled and anti-human IgM, alkaline phosphatase-labelled.
- EUROLINE Ganglioside Profiles allow the determination of up to 7 autoantibodies: antibodies against GM1, GM2, GM3, GD1a, GD1b, GT1b and GQ1b.
- The test strips can be automatically incubated and evaluated using the EUROBlotMaster or EUROBlotOne system and the EUROLineScan programme.
Neuronal ags profile
ANA Profile 3 (15 test parameters): Clinical association & prevalence
|
Antigens |
Disease |
Prevalence |
|
U1-nRNP |
MCTD SLE Rheumatoid arthritis |
95 – 100 % 15 – 40 % 3 % |
|
Sm |
SLE |
5 – 40 % |
|
SS-A (Ro) |
Sjogern’s syndrome SLE Neonatal Lupus syndrome |
40 – 95 % 20 60 % 100 % |
|
SS-B (La) |
Sjogern’s syndrome SLE |
40 – 95 % 10 – 20 % |
|
Scl -70 |
PSS, diffuse form |
25 – 75 % |
|
PM-Scl (PM-1) |
Poly-/ dermatomyositis / overlap syndrome PSS, diffuse form |
50 – 70 % 5 – 10 % |
|
Jo-1 |
Polymyositis / dermatomyositis |
25 – 35 % |
|
Centromeres (CENP.B) |
PSS, Limited form |
80- 95 % |
|
Cyclin (PCNA) |
SLE |
3 % |
|
Ds DNA |
SLE |
60 – 90 % |
|
Nucleosomes |
SLE |
40 – 70 % |
|
Histones |
Drug- induced LE SLE Rheumatoid Arthritis |
95 % 50 % 15 – 50 % |
|
Ribosomal P- proteins |
SLE |
10 % |
|
AMA-M2 |
Primary Biliary Liver Cirrhosis |
Ø 90 % |
Auto Immune profile:
|
ANA Profile 3 |
S.Sclerosis Profile |
Autoimmune Liver Profile 2 |
Myositis Profile 3 |
ANCA Profile |
Neuronal Antigen Profile2 |
Gangloside Profile 2 IgM, IgG |
|
nRNP/Sm |
SCL-70 |
AMA M2 |
Mi-2 |
Proteinase 3 |
Amphiphysin |
GM1 |
|
Sm |
CENP A |
M2-3E |
Ku |
MPO |
CV2 |
GM2 |
|
SS-A |
CENP B |
LKM-1 |
PM-SCL100 |
Elastase |
PNMA2 |
GM3 |
|
Ro-52 |
RP11 |
LC-1 |
PM-SCL75 |
Cathepsin G |
Ma-2 \ Ta |
GD1a |
|
SS-B |
RP155 |
SLA\LP |
Jo-1 |
BPI |
Ri |
GD1b |
|
Scl-70 |
FIBRILLARIN |
|
SRP |
Lactoferrin |
Yo |
GT1b |
|
PM-Scl |
NOR90 |
|
PL-7 |
|
Hu |
GQ1b |
|
Jo-1 |
Th\To |
|
PL-12 |
|
|
|
|
CENP B |
PM-Scl100 |
|
EJ |
|
|
|
|
PCNA |
PM-Scl75 |
|
OJ |
|
|
|
|
dsDNA-NcX |
Ku |
|
Ro-52 |
|
|
|
|
nucleosome |
PDGFR |
|
|
|
|
|
|
Histones |
Ro-52 |
|
|
|
|
|
|
Rib.P-prot |
|
|
|
|
|
|
|
AMA M2 |
|
|
|
|
|
|
Allergy Profile:
- Determination of allergen-specific IgE in serum
- Analysis of sensitizations against inhalation, food and insect venom allergens or cross-reactions
- Low amounts of serum sufficient (100-400 µl)
- Simultaneous detection of specific IgE against up to 54 single allergens within one incubation
- Customized profiles: Broad spectrum of indication- and region-specific profiles available
- Internal CCD marker enables differentiation between true poly-sensitizations and cross-reactivities due to carbohydrates
- Limited hands-on-time: fast and easy manual processing or highly efficient semi- and fully-automated processing using
EUROBlotOne, EUROBlotMaster and EUROLineScan.
Food & Inhalation Profile :
|
INHALATION PROFILE
|
FOOD PROFILE |
||
|
(g6, g12, t2, t3, t7, t9, w1, w6, d1, d2, i6, e1, e2, e3, e17, m1, m2, m3, m5, m6, CCD) |
(f1, f75, f2, f105, f4, f14, f45, fs36, f13, f92, f33, f44, f93, f25, f31, f48, f83, f88, f3, f23, CCD) |
||
|
g6 |
Timothy grass (عشب تموثي) |
f1 |
Hens egg white بياض البيض)) |
|
g12 |
Rye (عشب) |
f75 |
Hens egg yolk صفار البيض)) |
|
t2 |
Alder (عشب ألدر) |
f2 |
Cow’s milk proteinالبقر) بروتين لبن) |
|
t3 |
Birch (شجرة البتولا) |
f105 |
)شوكولاته) Chocolate |
|
t7 |
Oak (شجرة البلوط) |
f4 |
Wheat flour (دقيق القمح) |
|
t9 |
Olive (زيتون) |
f14 |
Soybean (فول الصويا) |
|
w1 |
Ragweed (عشب) |
f45 |
Bakerꞌs yeast S. cerevisiae (خيره الخبز) |
|
w6 |
Mugwort (عشب الميرميه) |
fs36 |
Nut Mix2 (Pea nut,Hazel nut, almond) مكسرات البندق واللوز والفول السوداني)) |
|
d1 |
Dermatophagoides pteronyssinus (عت غبار المنزل الاوربيه) |
f13 |
Peanut (الفول السوداني) |
|
d2 |
Dermatophagoides farina (عت غبار المنزل الامريكيه) |
f92 |
Nutmeg (جوز الطيب) |
|
i6 |
German cockroach (صرصور) |
f33 |
Orange (برتقال) |
|
e1 |
Cat epithelium (القط) |
f44 |
Strawberry (فراوله) |
|
e2 |
Dog hair (الكلب) |
f93 |
Mango (مانجو) |
|
e3 |
Horse epithelium (حصان) |
f25 |
Tomato (طماطم) |
|
e17 |
Camel hair + epithelium (الابل) |
f31 |
Carrot (الجزر) |
|
m1 |
Penicilium notatum (فطر) |
f48 |
Apple (تفاح) |
|
m2 |
Cladosporium herbarum (فطر) |
f83 |
Chicken (الدجاج) |
|
m3, |
Aspergillus fumigates (فطر) |
f88 |
Mutton /Lamb (لحم الضأن) |
|
m5 |
Candida albicans (فطر) |
f3 |
Codfish (سمك البحر) |
|
m6 |
Alternaria tenuis (فطر) |
f23 |
Crab ((سرطان البحر |
|
CCD |
CCD marker |
CCD |
CCD marker |
s.sclerosis profile