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Enzyme linked immunosorbent assay (ELISA) technique Course (Practical)

1. Course Name: ELISA technique

2. Course duration: 3 days (18 hours).

3. Course in perspectives: Immunology is one of the important sciences for medical laboratory students. It is an essential for understanding disease susceptibility, progression and diagnosis

4. Prerequisite is Basic immunology

5. Learning Objectives:

Describe and perform different types of Enzyme linked immunosorbent assay (ELISA) technique and it is Clinical application aspects.

6. Content

*Theory:

1. Reasons for Conduction

2. ELISA test mechanisms and types

3. Antigen design autoimmune (dsDNA-NcX, AMA-3E, Gliadin GAF3X)

4. Antigen design infectious (NS1 protein: Zika,Dengue virus, 5 Plasmodium species)

5. Diagnosis of zoonotic diseases (Camel:MERS-CoV IgG, Brucella ELISA IgG)

6. Troublesshooying in ELISA test systems

*Practical:

Intensive practical application for ELISA technique in Autoimmune, Infectious and Allergy diagnosis for 3 days.

7. Schedule:

Day

Lecture

Practical

1

* Overview of development of immunological Techniques

* ELISA technique

*EUROIMMUN ELISA innovation

Cardiolipin

2

-

Total IgE

3

-

EBV - EA